It was last minute heartbreak for The Doonhamers as Queens lost 2-1 to top of the league Rangers at Ibrox.
Derek Lyle gave Queens the lead but second half goals from Jason Holt and Marcus Waghorn made sure Rangers unbeaten start to the season continued.
After the victory against Morton last week, James Fowler made three changes to the starting line-up. Scott Hooper, Jake Pickard and on-loan Alex Harris started replacing the injured Lewis Kidd, Darren Brownlie and Andy Dowie.
Queens lined up with a 4-4-2 formation, Kyle Jacobs slotting into right back position with Scott Hooper and Chris Higgins in the centre of the back four.
Aidan Smith continued to partner up with Derek Lyle upfront and hoped to keep up his record of scoring against the Ibrox side. Smith scored in the same fixture last season and in the 5-1 defeat in August.
Rangers started strongly from the off and pegged Queens back into their own half. Rangers had an early chance when ex-Wigan striker Waghorn cross come shot was easily gathered by Robbie Thomson.
After the quick fire start to the game it died down with Queens soaking up the oncoming Rangers pressure without any real incident to note.
Queens were reduced to ten men for six minutes when Derek Lyle had to come off the pitch to receive medical attention for a gash on his forehead and with the numerical advantage Rangers began to cause Queens problems.
Lyle tries to get back onto the pitch but the linesman isn`t happy with the cut
Rangers captain Lee Wallace had a shot blocked which landed to the path of Waghorn but he couldn`t collect the ball as Hooper slid in well and cleared the ball.
The Rangers attacks kept on coming and after Barrie McKay brushed past Kyle Jacobs and cut the ball back to Kenny Miller it looked like the deadlock would be broken but the Rangers frontman drilled his effort over the bar.
On the 16th minute, against the run of play Queens mustered up their first chance of the day. Good link-up play from Ryan Conroy and Derek Lyle led in Alex Harris, whose pace left the Rangers defence for dead and his shot curled past Wes Foderingham but was just inches wide of the post.
Rangers were now dominating possession and with great link-up play between former Wigan players James Tavernier and Martyn Waghorn it fell to the leagues top goal scorer and he would have added another goal to his tally if it wasn`t for Robbie Thomson as he palmed the Englishmans shot wide.
Conroy helps out on the line
Waghorn must have thought it wasn`t going to be his day as he missed another chance to break the deadlock. Waghorn breezed past Chris Higgins and from 20-yards out he blazed his effort over the bar.
Rangers were playing nice creative football but weren`t taking advantage of the chances presented to them especially when Barrie McKay, who was causing problems for the Queens defence on the left wing all afternoon, cut the ball back to Rangers captain Lee Wallace who launched a long range effort at goal but was comfortably saved by Robbie Thomson.
Only minutes later Conroy earned Queens their first corner of the afternoon after his shot was deflected wide and from the corner Queens went ahead ten minutes before half-time.
Conroy whipped in the corner and met the head of Chris Higgins and the ball glanced across the six-yard box to Derek Lyle who simply tapped the ball into the back of the net for his fifth league goal of the season.
Lyle scores and turns away to celebrate......
...which he does in style with the Queens fans in the distant background
It was Queens` only shot on target in the first half but they made sure to make these chances count when they were presented with them.
Martyn Waghorn had a chance to immediately equalise but again Thomson was one step ahead and collected the ball to the frustration of the Ibrox crowd.
Rangers were pushing to level the match before half time but couldn?t find the final pass to unlock the makeshift Queens defence.
HALF TIME RANGERS 0-1 QUEENS
The second half started in the same fashion as the opening minutes of the first. When Kyle Jacobs was cautioned for bringing down McKay just outside the left hand side of the box.
Tavernier flashed a glorious free-kick goal bound but couldn`t find any Rangers player and went wide. Any touch and it could have been a level game.
Only a couple of minutes later Mark Warburton must have been thinking there was no way through for his side as Waghorn had a shot at goal but was denied by an incredible one handed save from Thomson.
Rangers kept coming back and Queens looked to counterattack the home side at every possible chance but Queens` resolve was finally broken on the 53rd minute.
James Tavernier`s through ball to Jason Holt was superbly timed and it gave the midfielder time to position his body and curve his effort from seven yards out into the bottom corner of the net, leaving Thomson no chance.
Holt finally beats Thomson
Rangers could have doubled their lead only moments later. Kenny Miller dinked the ball into the Queens penalty box and found the feet of McKay but he was off balance and his strike was well wide of the goal.
The Glasgow side were once again on top and were creating chances left, right and centre but couldn`t find the goal that would give them the lead for the first time in the match.
Waghorn tried his luck from 12 yards out but he placed his shot too high and once again couldn`t beat Robbie Thomson in the Queens net.
After a mix-up in the Rangers defence it gave substitute Liam Coogans the chance to give Queens the lead with 15 minutes to play. The striker on loan from Huddersfield, found himself one-on-one with Foderingham but Coogans fired his shot straight at the goalkeeper.
Coogans was trhough but unable to convert
The majority of the 44133 crowd at Ibrox were beginning to get frustrated as Rangers wasted another chance as Lee Wallace`s header flashed past the goal.
With two minutes remaining Andy Halliday was brought down in the box and referee Barry Cook pointed to the spot.
Penalty to Rangers
It was that man Martyn Waghorn who stepped up to take the penalty. Waghorn scored two penalties in the recent encounter at Palmerston.
This time however the penalty had a different outcome as Thomson guessed which way he would send it and made a superb save to the delight of the travelling Queens supporters who thought the penalty decision was harsh and justice had been served.
Robbie Thomson denies Waghorn
It looked like Queens would leave Ibrox with a share of the spoils but the game ended in the cruellest way for The Doonhamers.
Nathan Oduwa whipped in a cross from the left and found the head of Martyn Waghorn who glanced his header into the net and made sure all three points would stay in Glasgow.
Last minute agony for the visitors
It was heartbreak for Queens who were defensively impressive throughout the match. There were plently of positives to take from the game especially as Queens had several injuries to contend with and playing a team who have won their first ten league matches of the season.
Next week Queens are back at Palmerston Park against Livingston.