A penalty save from Rohan Ferguson and another strike from range from Wullie Gibson meant that Queens continued their fine run of form to maintain their place in the top four of the Championship.
The South were forced into one change from the side that won at Dens Park with Rhys McCabe replacing Nortei Nortey in the starting eleven. Dapo Mebude returned to the bench, as did Tommy Goss who missed out at Dundee due to injury.
The Pars were without their skipper Euan Murray, although had ex-Doonhamer Dom Thomas in their starting side, while Iain Wilson was on the bench.
It was a scrappy start to the game with Craig Wighton looking the most likely going forward. The visitors controlled much of the ball, although admittedly created very little.
The first-halves main talking points came after 10 minutes when James Maxwell was adjudged to be obstructing Wighton in the area. Mike Roncone pointed to the spot and up-stepped the leagues second top goalscorer in Declan McManus.
Wighton is awarded a penalty for a foul by Maxwell
But McManus was unable to find the back of the net with Rohan Ferguson making a terrific save low down to his right-hand side to keep the things level.
Penalty save by Ferguson
It was a sublime stop by the Queens goalie who has really stepped up his game in recent weeks, playing with a confidence about him.
The South very much struggled to gain much control in the opening period though. Isaiah Jones fired a shot that troubled Owain Fon Williams, but not enough to force much of a save out of the Welsh ?keeper.
The best chance of the opening period did fall Queens? way though. Ayo Obileye got on the end of a cross from the left from Jones, unleashing a left-foot volley that Fon Williams done brilliantly to parry away.
McManus tried his luck from the edge of the area just before half-time although his shot didn?t have enough bend on it to force its way inside the post.
HT: Queens 0-0 Dunfermline
A goalless opening period in which Ferguson was the star of the show, saving a McManus penalty.
The second-half got underway in a more urgent fashion with both sides looking to break the deadlock. Steven Whittaker volleyed wide from 18 yards before another long range effort caused Ferguson no problems.
But the deadlock was broken soon after on 50 minutes by the positively youthful Wullie Gibson.
Gibson celebrates his goal
A Jones cross worked its way to the edge of the area, with the ball coming nicely on to Gibson, 25 yards from goal, to strike a venomous effort past Fon Williams who arguably could have done better.
Dapo Mebude made his return to the first-team after 61 minutes, replacing Niyah Joseph, and was booked almost immeidately after preventing Fon Williams from clearing from a goalkick.
Euan East then had the chance to double Queens` lead on 67 minutes, although he was only able to guide his header wide of the post.
A myraid of change for the visitors saw the introductions of Iain Wilson, Kevin O`Hara and Scott Banks, although none of them really added much to a relatively poor away performance.
More bookings followed with Calvin McGrory, Gibson and Paul Watson seeing yellow, before Queens all but sealed the deal in the 87th minute.
It wasn`t a goal that sealed it either, rather a sending off for Lewis Mayo who was booked in the opening period. Mayo brought down his Rangers teammate Mebude as the tricky striker looked to run into the box, leaving referee Mike Roncone with no choice but to brandish a second yellow card.
Mayo sees his last action of the game as he fould Medube and picks up his second yellow of the match
The Pars really failed to exert any pressure on the Queens defence in the closing moments.
FT: Queens 1-0 Dunfermline
Yet another victory for a resurgant Queens and one that moves them just three points off of second in the table, albeit both Dunfermline and Raith have games in hand.
Next up it`s a trip to Gayfield as Arbroath host the Doonhamers.
Finlay Jack